Evidence of 5,000-year-old beer recipe found in China

Back in 2004, archaeologists excavated two pits in northern China that looked a lot like homebrewing operations. Constructed between 3400 and 2900 B.C. by the Yangshao culture, each pit contained the remnants of a stove and assorted funnels, pots and amphorae.

Now, Jiajing Wang of Stanford University and colleagues report that the pottery shards contain residue and other evidence of starches, chemicals and plant minerals from specific fermented grains. The ancient beer recipe included broomcorn millet, barley, Job’s tears and tubers — that probably gave the beer a sweet flavor, the team writes May 23 in the Proceedings of the National Academy of Sciences.

The findings predate the earliest evidence of barley in China by around 1,000 years. Beer may have been consumed at social gatherings, and brewing, not agriculture, spurred the introduction of barley to China, the researchers argue.

Readers share climate change concerns

Climate commotion
In “Changing Climate: 10 years after An Inconvenient Truth” (SN: 4/16/16, p. 22), Thomas Sumner reported on the progress scientists have made revising forecasts of the far-reaching effects of climate change — from extreme temperatures and sea level rise to severe drought and human conflict — in the decade since the Oscar-winning film’s release.

Reader response to the article was overwhelming, with hundreds of online comments. Some people enjoyed the in-depth look at climate change science, while others expressed skepticism about humans’ contribution to climate change and a general distrust of climate scientists.

“One of my goals for this article was to highlight that climate change research has itself changed over the last decade,” Sumner says. Scientists are still working to understand how the consequences of atmospheric warming will play out in the coming centuries. But one big message from the last decade of research is that the fundamentals have held up: Natural variability exists, says Sumner, but human activities are largely responsible for the current warming trend.
“The question now is what impact will human contributions have down the line and what should we do to prevent and mitigate those effects,” he says.
Plastic feast
Sarah Schwartz wrote about the discovery of a microbe, Ideonella sakaiensis, that chows down on a hard-to-degrade polymer in “This microbe makes a meal of plastic” (SN: 4/16/16, p. 5).
Online commenters were amazed by this new plastic-gobbling organism. “This is great news,” Dan said. “Our world would be doomed if there wasn’t a microbe able to do this.” Chuckawobbly wondered how long it takes I. sakaiensis to digest the plastic. And Jean Harlow was concerned about the potential by-products of worldwide plastic digestion. “The waste product would be a significant amount … of what?” she asked.

Researchers observed that I. sakaiensis almost completely degraded a thin film of polyethylene terephthalate, or PET, after six weeks in a laboratory. But when extracted from the bacterium, the proteins used to break down plastic begin working in about 18 hours.

I. sakaiensisappears to break PET into smaller molecules, like amino acids and carbon dioxide, says coauthor Kenji Miyamoto of Keio University in Yokohama, Japan. But it would probably be hard for the microbe to break down plastic in the outdoors because of its specific growth requirements, he says. Miyamoto envisions that it could be possible to use the specialized proteins in a closed environment to break PET down into molecules such as terephthalic acid— one of the plastic’s main building blocks, which seems benign in the environment.

Prairie dog predators
Herbivorous prairie dog mothers routinely kill baby ground squirrels that encroach on their territories, researchers found. Competition for resources may be a contributing factor to the killings, Susan Milius reported in “Killer prairie dogs make good moms” (SN: 4/16/16, p. 14).

One reader had other ideas. Audrey Boag wondered if prairie dog moms kill ground squirrels to protect their pups from predation or from diseases carried by the squirrels. “In either case, minimizing the number of ground squirrels would pay in lifetime biological fitness,” she wrote.

“We never observed a ground squirrel kill or injure an adult or juvenile prairie dog,” says study coauthor John Hoogland. “Perhaps such attacks sometimes occur underground.” Hoogland notes that the majority of ground squirrels killed by prairie dogs were juveniles, which are too small to be a threat.

One threat, however, is a species of disease-carrying flea that infests both animals. Hoogland found that prairie dog killers and their offspring had fewer fleas than nonkillers and their offspring, “but this trend was not significant,” he says.

Biologists seek help to ‘see’ itty-bitty molecules in 3-D

Microscopy Masters asks one thing of citizen scientists: Find proteins in electron microscope images. The task will probably give participants new appreciation for biologists who decipher the structures of teeny, tiny molecules. It’s not easy.

The goal of the online project, created by researchers at the Scripps Research Institute in La Jolla, Calif., is to improve biologists’ ability to construct detailed, three-dimensional models of proteins.

Using cryo-electron microscopy — which involves freezing, then imaging a sample — the researchers have taken thousands of photos of their current target, a protein complex involved in breaking down other, unwanted proteins. Each image contains 10 to 100 copies of the complex. It takes that many images to capture a protein from every angle. Once the 2-D images are stitched together, researchers can reconstruct the protein’s globular, 3-D shape at near-atomic resolution.
Microscopy Masters enlists volunteers to do the necessary first step of combing through the photos to find the protein molecules — a time-consuming job that people do better than computers. The task may feel daunting, as each black-and-white image resembles a fuzzy TV screen. Only some of the dark smudges in any given image will be molecules of interest; others will be actual smudges or globs of proteins too jumbled to be of use. Fortunately, a practice tutorial offers a crash course in protein identification. And each image will be classified by many users, alleviating some of the pressure of worrying about marking the wrong thing.

Data from the project will help researchers improve protein-picking computer algorithms, says project member Jacob Bruggemann. That way computers can take over the painstaking work.

Bulging stars mess with planet’s seasons

SAN DIEGO — On some planets that orbit whirling stars, spring and autumn might be the best time to hit the beach, whereas summer offers a midyear respite from sweltering heat. These worlds’ orbits can take them over regions of their sun that radiate wildly different amounts of heat.

“Seasons on a planet like this must be really strange,” says Jonathon Ahlers, a graduate student at the University of Idaho in Moscow, who presented his findings June 15 at a meeting of the American Astronomical Society.
Some stars spin so fast that they bulge in the middle. That bulge pushes the equator away from the blazing core, making it much cooler than the poles. A fraction of these stars also host planets that travel on cockeyed orbits, which take these worlds alternately over the poles and equator of their sun.

Ahlers developed computer simulations to see how the differences in solar energy combined with the tilted orbits might affect a planet’s seasons. The outcome depends on how the planet’s axis is tipped relative to its orbit. For a world whose north and south poles periodically face the star’s equator, “you get a cooler summer than normal and an extremely cold winter, but spring and autumn can be hotter than summer,” says Ahlers. “You get two distinct hottest times of the year.”

How that plays out depends on how the planet is built: an atmosphere or oceans could mitigate climate extremes. Ahlers has yet to work out those details. “It’s doing a lot,” he says, “but what, I don’t really know yet.”

Jupiter shows off its infrared colors

No, that’s not the sun. It’s Jupiter, ablaze with infrared light in new images taken in preparation for the Juno spacecraft’s July 4 arrival at the king of the planets. This image shows how heat welling up from deep within the planet gets absorbed by gas in the atmosphere, which can tell researchers how stuff moves around beneath Jupiter’s thick blanket of clouds. Juno won’t look for infrared light, but it will (among other things) measure how much microwave radiation is being blocked by water lurking within Jupiter’s atmosphere.

The map is pieced together from multiple images obtained at the Very Large Telescope in Chile over the past several months. Ground-based images such as these will help researchers understand what Juno is peering at each time it swoops in close to Jupiter’s clouds over the next 20 months.

IVF doesn’t up long-term breast cancer risk, study says

For women thinking about fertility treatments, there may be one less thing to worry about.

A long-term study shows that women who underwent in vitro fertilization are not significantly more likely to develop breast cancer than women in the general public or women who opted for other fertility treatments. The results are reported July 19 in JAMA.

The fertility treatment alters progesterone and estradiol levels in women trying to get pregnant. Yo-yoing hormones have been linked to an increase in a woman’s odds of developing breast cancer, but studies are divided on whether IVF itself actually ups cancer risk.

Alexandra van den Belt-Dusebout of the Netherlands Cancer Institute in Amsterdam and her colleagues tracked 19,158 women who underwent in vitro fertilization treatment between 1983 and 1995 and 5,950 women who underwent other fertility treatments between 1980 and 1995.

Following up two decades later, the team found that 948 of the women had developed breast cancer. But breast cancer rates didn’t differ much between groups: 163.5 per 100,000 women for those who had IVF compared to 167.2 women on other fertility treatments and 163.3 women in the general public.

Ancient reptiles saw red before turning red

You’ve got to see it to be it. A heightened sense of red color vision arose in ancient reptiles before bright red skin, scales and feathers, a new study suggests. The finding bolsters evidence that dinosaurs probably saw red and perhaps displayed red color.

The new finding, published in the Aug. 17 Proceedings of the Royal Society B, rests on the discovery that birds and turtles share a gene used both for red vision and red coloration. More bird and turtle species use the gene, called CYP2J19, for vision than for coloration, however, suggesting that its first job was in sight.
“We have this single gene that has two very different functions,” says evolutionary biologist Nicholas Mundy of the University of Cambridge. Mundy’s team wondered which function came first: the red vision or the ornamentation.

In evolution, what an animal can see is often linked with what others can display, says paleontologist Martin Sander of the University of Bonn in Germany, who did not work on the new study. “We’re always getting at color from these two sides,” he says, because the point of seeing a strong color is often reading visual signals.

Scientists already knew that birds use CYP2J19 for vision and color. In bird eyes, the gene contains instructions for making bright red oil droplets that filter red light. Other forms of red color vision evolved earlier in other animals, but this form allows birds to see more shades of red than humans can. Elsewhere in the body, the same gene can code for pigments that stain feathers red. Turtles are the only other land vertebrates with bright red oil droplets in their eyes. But scientists weren’t sure if the same gene was responsible, Mundy says.

His team searched for CYP2J19 in the DNA of three turtle species: the western painted turtle, Chinese soft-shell turtle and green sea turtle. All three have the gene. Both birds and turtles, the researchers conclude, inherited the gene from a shared ancestor that lived at least 250 million years ago. (Crocodiles and alligators, close relatives of birds and turtles, probably lost the gene sometime after splitting from this common ancestor, Mundy says.)

Next, the scientists turned their attention to the gene’s function. Mundy’s team studied western painted turtles, which have a striking red shell. As in red birds, CYP2J19 is active in the eyes and bodies of these turtles, the scientists found, suggesting that the gene is involved in both vision and coloration.
Because most birds and turtles can see red, but only some have red feathers or scales, the researchers think the great-granddaddy of modern turtles and birds probably used the gene for vision, too. Whether that common ancestor was colored red is unclear.

That very old reptile would have passed CYP2J19 down to its descendants, including dinosaurs. Mounting evidence has pointed to dinosaurs as colorful, with good color vision. But the specifics of their coloration have been elusive. This study points to red as one color they could probably see, and perhaps display.

“If you would have asked me 10 years ago, ‘Will we ever know the color of dinosaurs?’” Sander says, “I would have said, ‘No way!’” But studies like this one are a new lens into dinosaur color. Seeing can mean displaying, and this study is solid evidence that dinosaurs saw red, Sander says. In the past, “we couldn’t really say that.”

Supersmall device uses individual atoms to store data

These orderly patterns of dark blue dots indicate where individual chlorine atoms are missing from an otherwise regular grid of atoms. Scientists manipulated these vacancies to create a supersmall data storage device.

The locations of vacancies encode bits of information in the device, which Sander Otte of Delft University of Technology in the Netherlands and colleagues describe July 18 in Nature Nanotechnology. The team arranged and imaged the vacancies using a scanning tunneling microscope. The storage system, which can hold a kilobyte of data, must be cooled to a chilly −196° Celsius to work.
To demonstrate the technique, the researchers transcribed an excerpt from a famous 1959 lecture by physicist Richard Feynman, “There’s Plenty of Room at the Bottom,” which predicted the importance of nanotechnology. In each block, paired rows represent letters. Blocks marked with an “X” were unusable. The encoded 159 words of text fill a region a ten-thousandth of a millimeter wide.

If scaled up, the researchers say, the technology could store the full contents of the U.S. Library of Congress in a cube a tenth of a millimeter on each side.

High-tech cloth could make summer days a breeze

Plastic cling wrap with nano-sized pores could give “cool clothes” a new meaning.

The material lets heat escape, instead of trapping it like traditional fabrics, Stanford University materials scientist Yi Cui and colleagues report in the Sept. 2 Science. It could help people keep cool in hot weather, Cui says, and even save energy by reducing the use of air conditioning.

“It’s a very bold new idea,” says MIT physicist Svetlana Boriskina, who wrote an accompanying commentary. Demand for the new material could be far-reaching, she says. “Every person who wears clothes could be a potential user of this product.”
Current cooling devices include wearable fans and wicking fabrics; both rely on evaporation to cool human skin. But skin also sheds heat in another way — as infrared radiation. Clothing holds this heat close to the body, Cui says. If infrared radiation could instead pass through fabric, he reasoned, people would feel a lot cooler.

But the fabric would have to be transparent only to infrared wavelengths. To visible light, it would need to be opaque. Otherwise, the clothing would be see-through.

Cui found just one material that satisfied both requirements: a commercially available plastic used in lithium-ion batteries. The material, called nanoporous polyethylene, or nanoPE, is a cling wrap‒like plastic that lets infrared radiation through. But unlike cling wrap, the material isn’t clear: It blocks visible light.

Tiny pores speckled throughout the fabric act as obstacles to visible light, Boriskina says. When blue light, for example, hits the pores, it scatters. So do other colors. The light “bounces around in different directions and scrambles together,” she says. To human eyes, the resulting color is white.

The pores scatter visible light because they’re both in the same size range: The diameters of the pores span 50 to 1,000 nanometers, and the wavelengths of visible light range from 400 to 700 nanometers. Infrared light emitted by the body has a much larger wavelength, 7,000 to 14,000 nanometers, so the plastic’s tiny pores can’t block it. To infrared light, the pores are barely bumps in the road, not barriers.
The pores are kind of like small rocks at a beach, Boriskina says. They’ll interfere with the motion of small waves, but big waves will wash right over.

Cui and colleagues tested nanoPE by laying it on a hot plate warmed up to human skin temperature — 33.5° Celsius. NanoPE raised the “skin” temperature by just 0.8 degrees(to 34.3° C). “But when you put on cotton, my God, it rose to 37,” Cui says. “It’s hot!”

The researchers also tried to make nanoPE more wearable than plastic wrap. They coated it with a water-wicking chemical, punched holes in it to make it breathable, and layered it with cotton mesh. Now, the team is working on weaving the fabric to make it feel more like traditional textiles.

“Within five years, I hope someone will start wearing it,” Cui says. “And within 10 years, I hope most people will be wearing it.”

Digital rehab exposes Biblical roots of ancient Israeli scroll

Researchers have digitally unwrapped and read an ancient Hebrew scroll that’s so charred it can’t be touched without falling apart. It turns out the document contains the oldest known Biblical text outside of the roughly 2,000-year-old Dead Sea Scrolls, the investigators say.

Archaeologists discovered the scroll’s remnants in a synagogue’s holy ark during a 1970 excavation in Israel of En-Gedi, a Jewish community destroyed by fire around 600.

In a series of digital steps, slices from a 3-D scan of the En-Gedi scroll were analyzed to bring letters and words into relief on a pieced-together, virtual page. Those images revealed passages from the book of Leviticus written in ink on the scroll’s disintegrating sheets. Radiocarbon results date the scroll to approximately 300, making it the earliest copy of an Old Testament book ever found in a holy ark, scientists report September 21 in Science Advances.

This computerized recovery and conservation process can now be used to retrieve other ancient documents “from the brink of oblivion,” the researchers say.